High prevalence of B and G aflatoxin-producing fungi in sugarcane field soil in Japan: heteroduplex panel analysis identifies a new genotype within Aspergillus Section Flavi and Aspergillus nomius
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چکیده
منابع مشابه
Sexual reproduction in aflatoxin-producing Aspergillus nomius.
Sexual reproduction was examined in the aflatoxin-producing fungus Aspergillus nomius. Crosses between sexually compatible strains resulted in the formation of multiple nonostiolate ascocarps within stromata, which places the teleomorph in genus Petromyces. Ascocarp and ascospore morphology in Petromyces nomius were similar to that in P. flavus and P. parasiticus, and differences between teleom...
متن کاملHeteroduplex panel analysis, a novel method for genetic identification of Aspergillus Section Flavi strains.
For genetic identification of Aspergillus Section Flavi isolates and detection of intraspecific variation, we developed a novel method for heteroduplex panel analysis (HPA) utilizing fragments of the internal transcribed spacer (ITS) regions (ITS1-5.8S-ITS2) of the rRNA gene that was PCR amplified with universal primers. The method involves formation of heteroduplexes with a set of reference fr...
متن کاملTwo new aflatoxin producing species, and an overview of Aspergillus section Flavi
Aspergillus subgenus Circumdati section Flavi includes species with usually biseriate conidial heads, in shades of yellow-green to brown, and dark sclerotia. Several species assigned to this section are either important mycotoxin producers including aflatoxins, cyclopiazonic acid, ochratoxins and kojic acid, or are used in oriental food fermentation processes and as hosts for heterologous gene ...
متن کاملEvaluating Aflatoxin Gene Expression in Aspergillus Section Flavi.
The determination of aflatoxin production ability and differentiation of aflatoxigenic strains can be assessed by monitoring the expression of one or several key genes using reverse transcription polymerase chain reaction (RT-PCR). We herein describe the methods for RNA induction, extraction, and quality determination, and the RT-PCR conditions used to evaluate the ability of a given Aspergillu...
متن کاملA Survey on Contamination of Iranian Pistachio Cultivars to Aspergillus Section Flavi and Aflatoxin
Population density of Aspergillus section Flavi in pistachio kernels of Iranian cultivars was studied in main pistachio growing areas of Iran during 2015 to 2017. In order to investigate the rate of contamination of pistachio cultivars to Aspergillus section Flavi and aflatoxins, 13 pistachio cultivars, including Akbari, Kaleh-ghouchi, Ovhadi, Ahmad-aghaee, M...
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ژورنال
عنوان ژورنال: FEMS Microbiology Ecology
سال: 2003
ISSN: 0168-6496,1574-6941
DOI: 10.1016/s0168-6496(03)00154-5